Bd lsr ii users guide describes procedures necessary to operate and maintain your bd lsr ii flow cytometer. Bd facsuite software provides an easytouse yet flexible interface for data acquisition and analysis with the bd facslyric system. To log into the computer, use lsrii as the user name and lsrii as the password. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Lsr iib and lsriic turn on the fluidics pump and ensure that it is running. Bd lsr ii users guide indiana university bloomington. Check that the air line and the blue fluidics line is connected to the sheath tank. Each sale of a stored unit of this software grants the purchaser a nontransferable, nonexclusive, personal license. Firsttime users of bd facsdiva software should read. As well as acquiring your data using diva software, you can also analyse your data after you have run your samples. It is the responsibility of the buyeruser to ensure that all added electronic files including software. See headers and footers on page 100 in the bd facsdiva software reference manual.
Can anyone recommend a good free facs analysing software. Together with 4k content and a 4k display, you could download windows bluray player freeware securely download free software. Bd, bd logo, and all other trademarks are property of becton, dickinson and company. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Feb 25, 20 how to use experiment layout in facsdiva raffaello cimbro. Unless otherwise noted, bd, the bd logo, and all other trademarks are property of becton.
Add 250 ml of bleach to the empty waste tank if necessary. Compensation of a 7 color panel on the bd lsr ii uc merced scif stem cell instrumentation foundry. Xshift, hyperfinderandcluster explorer commercially available since 1996, flowjo is part of bd since october 2017 background. Im watching the tutorial and it seems very interesting and easy to use. I dont have an lsr, but an ariaii so some of the specifics will be different, but the principles the same. The most popular version of this product among our users is 4. The wide range of product courses includes handson, instructorled training, as well as elearning in instructor led virtual classrooms, selfpaced study, and online webinars. If you are an experienced user, try the updated tutorials to. Modeling the distribution of wild peanuts arachis spp. If you are the last user for the day, please perform the machine shut down procedure as laid out.
Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. The name of the program executable file is bd facsdiva software. This manual describes how to use bd facsdiva software. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample. Aug 25, 2017 compensation of a 7 color panel on the bd lsr ii uc merced scif stem cell instrumentation foundry. Turn on the instrument using the green power button. Ive been using bd s cell cycle test plus kit and i am. Topro3 parameter setup the parameter setup when using topro3 is as described above but using the apc 66020 nm channel on the bd lsrii and the bd facscanto ii. This video describes how to set up an experiment in facs diva version 8.
Exports fcs files that can be imported into diva, flowjo or other flow analysis software. Bd facsdiva software reference manual for version 7. I only came upon this after our bd service engineer sent me a copy. Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. Expand the specimen and make the tube active by clicking the arrow next to the tube. Some help with climate data, niche modeling, and clm files. Because many cytometer functions are controlled by bd facsdiva software, this guide also contains information about software features required for basic cytometer setup and operation. You can change gates and plots, add statistics and create pdf files. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. Phi, pii in accordance with all applicable federal and state privacy and security laws, including the health insurance portability and. Allow the instrument to connect to the software if necessary. Flowjo is your biggest fan and strives to be an outstanding source of support. Download bead lot files to support cell analysis using bd facsdiva software. First, bd has a diva software tutorial you should have gotten a copy with the instrument.
Sep 23, 2019 i got the bd technical bulletin called standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software which describes how to make two or more machines. Bd facsdiva software overview selfpaced data display in bd facsdiva software selfpaced using the worksheet window in bd facsdiva software selfpaced using templates in bd facsdiva software selfpaced bd facsvia online training program. Bd facs canto ii flow cytometer quick user guide v. Turning data into results load data analyze export results for research use only.
If you are an experienced user, try the updated tutorials to become familiar with new software features. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Bd facsdiva software features bd facsdiva software bd. This software may not be duplicated, reproduced, or copied in any form or by any means whatsoever, except.
In the login window, scroll down through the list of user names to locate yours. Refer to the information supplied by the manufacturer for applicationspecific limitations. Mar 05, 2020 bd, the bd logo and all other trademarks are property of becton, dickinson and company. Cell cycle tutorial queen mary university of london. Bd biosciences facsuite software features setup and. Were here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to results. Apr 03, 2014 cytometry on air all things facsdiva ryan duggan. Sophie novault bd facs diva software research institut.
Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. Bd facsuite software minimizes the number of user interactions with the instrument so that the user can spend more time working with samples. This product includes software developed by the apache software foundation. The application is designed for userdefined assays and collaboration through the assay portability feature, and provides a simple, reproducible and transferrable setup. Download the tutorial pdf and the accompanying data. Flow cytometry service bd lsr fortessa sorp how to use guide 6 14 in the cytometer panel, in the parameters tab, adjust the voltages of fsc and ssc in order to roughly place your cells of interest at the center of the dotplot. Bd facsdiva software standardizing application settings across multiple cytometers. Bd is committed to providing safe and secure products to our customers given the important benefits they provide to patient health. Use requires a sufficient background and expertise in mathematics and objective analysis. Running a basic 2 color flow cytometry experiment in bd facs diva duration.
Our selfpaced elearning courses can be used for basic learning or to refresh and reinforce what you learned in class. Bd facsdiva software reference manual for version 8. The compensation process works better if becton dickinson compensation beads are used as the automated facsdiva software algorithm has to reference nonfluorescent material, thus the use of unstained cells will give incorrect compensation values, which can be corrected manually. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Index sorting from facsdiva to flowjo flow cytometry news. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. For information on how to operate and maintain your flow cytometer, see your cytometer manual. Compatibility with diva acquisition software, plugins from bd. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently.
The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Running a basic 2 color flow cytometry experiment in bd facs diva. This guide contains instructions for using bd facsdiva software version 6. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password. If you use a becton dickinson bd cytometer in your lab, the chances are you are acquiring your data using diva software. You can change gates and plots, add statistics and. The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation.
Bd facscanto ii system overview bd facsdiva software v6. If you are not familiar with the windows operating system, see the documentation provided with your computer. For information on how to operate and maintain your flow cytometer, refer to the instrument users. Mar 01, 2015 the first, oddly enough, is bd s very own index sorting manual software manual and may not even be installed on your computer or available for download from bd s website. After a successful login, the bd facsdiva workspace appears showing the main application windows which include browser, cytometer, inspector, acquistion dashboard and global worksheet. Bd biosciences delivers software and workstations that are intended for running the instruments supplied by bd biosciences. Bd facsdiva software download 15 download ad3dc120ad over 100 of the best pc programs. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Bd facsdiva software resources software bd biosciences. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software training. Bd lsr fortessa standard operation protocol refill sheath container 1.
The first, oddly enough, is bd s very own index sorting manual software manual and may not even be installed on your computer or available for download from bd s website. This software is the property of becton, dickinson and company. Once you become familiar with routine operation and need only a quick reminder of the software menus or keyboard shortcuts, see appendix a. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an experienced user. I make all my users do it on their own time as part of our training. Next you want to tell the software what populations to show in each plot. This software may not be duplicated, reproduced, or copied in any form or by any means whatsoever, except as otherwise permitted by law. Disconnect the air line green and fluid line blue from the sheath container. If you are a new user, use the tutorials to get started with the software. Most software functions are controlled using the tool bar at the top of the workspace and toolbars within the browser and worksheet windows.
This opens a new window with the labels tab at the top of the window. Unless otherwise noted, bd, the bd logo and all other trademarks are property of becton. Preparing a facsdiva exp10 university of rochester. Tutorial bd high throughput sampler option for bd lsr ii after completing this tutorial, you will be able to. Training resources and tools bead lot files bd horizon guided panel solution gps tool panel design education bd biosciences resource library. I only came upon this after our bd service engineer sent me a copy of it. Running a basic 2 color flow cytometry experiment in bd. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an. To help customers take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications. We value the confidentiality, integrity and availability of all protected health and personally identifiable information e. Here well have a discussion covering many topics relating to the bd facsdiva platform including software and hardware. Diva online application and visualisation finally, for using simple diva tools from within odv, you can download the latest odv version with diva support odv software with diva integrated diva is fit for use on windows and unix platforms.